Project
Part:BBa_K2056004:Design
Designed by: Muhammad Ismail Group: iGEM16_Peshawar (2016-10-13)
NOx(Oxides of Nitrogen) sensor device (pNirK-Yellow chromoprotein)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 220
Illegal AgeI site found at 1116 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1493
Design Notes
The selection of the RBS and Constitutive promoter. Because of limitations of repeats that could be synthesized, we had to give up on the double terminators in the original design and go with single different terminators. A 70bp spacer(B0040) has been added for transcriptional efficiency.
Source
All parts in this sequence come from the Registry and have been listed. However, the complete part has been synthesized from IDT using their free gBLocks offer.